Vol. 5, Issue 1, Part B (2018)

Author(s): Mohammed M, Abakar AD, Bakri YM Nour and Dafalla OM

Abstract: Dengue fever is considered to be the most important mosquito-borne disease and considered as endemic disease in Jazan region, Saudi Arabia. The present study was aimed to analyze the prevailing dengue virus serotypes in Sabya governate of Jazan region. 100 Serum samples of NS1 positive cases samples were collected from November 2015 to October 2016 and tested by one step Reverse Transcription Polymerase Chain Reaction (RT-PCR) with a set of specific primers for detection of four dengue virus serotypes followed by sequencing the PCR products to confirm the results. Out of the 100 serum samples, 33 were found positive for dengue infection (33.0%). Two dengue virus serotypes were detected; DEN-2 and DEN-3. DEN-2 is the most common and predominant type in tested samples 69.7% (23/33) and then DEN-3 30.3% (10/33). The high seroprevalence of dengue virus infections in Sabya governate indicates its endemicity. The present study highlights the importance of tracking the spread of dengue virus types and its implication for analyzing changes in dengue endemicity in specified areas over time. Complete genome sequencing is required for the tow detected dengue virus in the governate (DEN-2 and DEN-3) to serve as references for any future epidemiological researches and/or outbreaks.

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