Vol. 11, Issue 5, Part B (2024)

Author(s): Sanjukta Mandal and Goutam Chandra

Abstract: An attempt was made to establish the larvicidal activities of Jacquinia ruscifolia green leaf extracts against third instar larvae of Culex quinquefasciatus under laboratory and semi-field conditions along with isolation and identification of bioactive compounds responsible for the larvicidal activity. Dose-dependent mortality was performed through graded concentrations with crude leaf extract (0.5, 1.0, 1.5, 2.0 and 2.5%), chloroform: methanol solvent extract (50, 100, 150, 200 and 250 ppm) and TLC fraction (50, 60, 70, 80 and 90 ppm) under laboratory condition. The highest larvicidal mortality was observed in 2.50% crude concentration, 250 ppm chloroform: methanol solvent extract and 90 ppm TLC fraction at 72 h of exposure. The lowest LC50 value 22.44 ppm was observed in the case of bioassay with solvent extract at 72 h of exposure. The results obtained in the semi-field suggest the lowest LC50 value is 37.30 at 72 h of exposure. FTIR analysis suggests the presence of functional groups, viz., ketone (=CO-), ether (O=), amines (-N=), alkanes (CnH2n+n) and alcohols (HO-R-OH group). GC-MS analysis suggests the presence of four active compounds viz.; Dipiperidino disulfide, 3-methyl-2-butenoic acid, Urea 1-methyl cyclopropyl and Bicyclo (2,2,2) octenone. Jacquinia ruscifolia offers a potential mosquito larvicidal agent against Cx. quinquefasciatus larvae. Previously, there is no recorded work on J. ruscifolia that can help us to compare the efficiency of this botanical extract. By taking it as a novel source there is a lot of scope to use it as mosquito larvicide in future.

DOI: 10.22271/23487941.2024.v11.i5b.814