Resistance profile of Aedes aegypti (L.), towards commonly used insecticides in Jeddah Governorate, Kingdom of Saudi Arabia

Youssif Elsamani Ibrahim; Hind Abdelaziz Elnasri; Mona Abdelrahman Mohamed Khaier; AT Alikhan Masroor; ELTayab Mohamed Elamin Abu ELZein

doi:10.22271/23487941.2024.v11.i5a.806

Resistance profile of Aedes aegypti (L.), towards commonly used insecticides in Jeddah Governorate, Kingdom of Saudi Arabia

Author(s): Youssif Elsamani Ibrahim, Hind Abdelaziz Elnasri, Mona Abdelrahman Mohamed Khaier, AT Alikhan Masroor and ELTayab Mohamed Elamin Abu ELZein

Abstract: Dengue fever is prevalent in western Saudi Arabia since 1990. Dengue fever vector Aedes mosquito is mostly control by different chemical insecticides Aedes vector developed resistance to different insecticides as reported from different parts of the world. The effectiveness of vector control is reduced. Little information is available in the study area about the insect resistance to Aedes mosquitoes which are vector for Dengue fever virus. Present research work was taken to study the presence and degree of resistance to commonly used insecticides in Saudi Arabia to control the vectors and to understand the mechanism of physiological resistance to adult mosquitoes Aedes aegypti.

WHO recommended tube assay method were applied to observe mortality and to investigate the insecticide resistance in Aedes mosquitoes. WHO resistance bioassay of mosquitoes with Deltamethrin (0.05%), Malathion (5%), Bendiocarb (1.0%) and Permethrin (0.75%), was conducted. To detect the target site mutation Polymerase chain reaction (PCR) technique was used.

Present study indicated that the exposure of adult Aedes female mosquito to the diagnostic doses Permethrin (0.75%), Deltamethrin (0.05%), Malathion (5%), and Bendiocarb (1.0%) caused 11,49,61.99 and 23% mortality respectively show resistance to these insecticides.

Multiple knocks down resistance (Kdr) Mutations are found in local population of Aedes aegypti in Jeddah region associated with Pyrethroid resistance. DNA sequencing for Voltage gated sodium channel (VGSC) was done on Aedes aegypti collected from different points of Jeddah for the presence of Kdr mutations. The present study revealed mutations at codon 989, 1016 and 1534.

These findings showed that the Aedes aegypti population has developed resistance against Pyrethroid insecticides. There is an urgent need to find better solution for the mosquito control.

DOI: 10.22271/23487941.2024.v11.i5a.806

Pages: 56-63 | 292 Views 119 Downloads

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Vol. 11, Issue 5, Part A (2024)

Resistance profile of Aedes aegypti (L.), towards commonly used insecticides in Jeddah Governorate, Kingdom of Saudi Arabia

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