CONTACT: +91-9711224068
International Journal of Mosquito Research
  • Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal
International Journal of Mosquito Research
Vol. 9, Issue 1, Part B (2022)

In vitro antiplasmodial and in vivo toxicity potential of Mentha piperita and Ocimum gratissimum essential oils and their synergistic effect with conventional antimalarial drugs against Plasmodium falciparum

Author(s): Chude Chidimma Florence, Nwankwo Edith Nonye and Ezekwesili-Ofili Ozioma Josephine
Abstract: The study aimed to investigate the in vitro antiplasmodial and in vivo toxicity potential of essential oils from Mentha piperita and Ocimum gratissimum against Plasmodium falciparum, singly and in combination with antimalarial drugs. The essential oils (EOs) were obtained by steam distillation and phytochemical screening conducted using gas chromatography equipped with a flame ionization detector. Blood samples of malaria positive patients were cultured and P. falciparum identified microscopically. Micro-technique of schizont counting was used to determine the antiplasmodial baseline of the EOs and in combination with antimalarial drugs. Hematological and serum biochemical indices for in vivo toxicity assay using Wister rats were determined following WHO test procedures. Dose-response assay determined after 48 h for in vitro inhibition of maturation of trophozoites into schizonts showed that EO of M. piperita had a higher antiplasmodial activity of 6.62 ± 0.20, 10.74 ± 1.64 and 14.21 ± 0.45 µg values than O. gratissimum for IC30, IC50 and IC90, respectively against P. falciparum. When the EOs were combined with conventional antimalarial agents, M. piperita with Sulfadoxine/Pyrimethamine had the highest antiplasmodial activity with inhibitory concentration values of 1.92 ± 1.00, 4.07 ± 1.34 and 6.85 ± 0.75 ݜ‡g/mL for IC30, IC50 and IC90, respectively than O. gratissimum. In vivo hematological and biochemical studies after 14 days oral treatment showed no toxicity as a result of treatment with the oil extracts and these were significantly different (P< 0.05). The study suggests that EOs of M. piperita and O. gratissimum could act individually or synergistically with conventional antimalaria drugs as effective alternative drug candidate in human malaria treatment.
Pages: 114-122  |  114 Views  8 Downloads
How to cite this article:
Chude Chidimma Florence, Nwankwo Edith Nonye, Ezekwesili-Ofili Ozioma Josephine. In vitro antiplasmodial and in vivo toxicity potential of Mentha piperita and Ocimum gratissimum essential oils and their synergistic effect with conventional antimalarial drugs against Plasmodium falciparum. Int J Mosq Res 2022;9(1):114-122. DOI: https://doi.org/10.22271/23487941.2022.v9.i1b.589
International Journal of Mosquito Research