Abstract: In the past ten years, Madurai District has been experiences of severe epidemics of dengue. These outbreaks coincided with a high increase in populations of Ae. albopictus that assists its sister taxon Ae. aegypti in most areas sampled. The goal of this work was to analyze the entomological investigation of the two Aedes species in Madurai district which has to face these arboviroses. The study area of Madurai District lies between 9°30’ and 10° 50’ of North latitude and from 77°00’ to 78° 30’ of East longitude. The purpose of selection of the study area is to investigate the spatial distribution of Aedes female mosquitoes and to analyze various factors, for example, mosquito sex, composition, rural and urban dissemination of selected species, container wise breeding and seasonal breeding are related and grouped in a heat map. The study was mainly based on primary data. The heat map grouping were made dependent on the sampling of Aedes mosquitoes collected for one year, from March 2017 – Feburary 2018 in dry and wet season in seven taluks of Madurai district in both rural and urban areas. Arc GIS used to analyze female per positive houses and to identify Aedes mosquitoes breeding variation. Principal Component Analysis, (PCA) used to explore the cluster of sex and composition of mosquitoes in rural and urban regions in dry and wet season.