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International Journal of Mosquito Research
Vol. 6, Issue 6, Part B (2019)

Molecular characterization and malaria transmission potential of Anopheles gambiae complex in Awka, Anambra state, Nigeria

Author(s): Irikannu KC, Onyido AE, Umeanaeto PU, Onwube O, Ogaraku JC, Egbuche CM, Oyeniyi AT, Adesalu OO and Jimoh TR
Abstract: The molecular characterisation and malaria transmission potential of Anopheles gambiae complex mosquitoes in Awka, Anambra State were studied from June to September, 2018. Indoor and outdoor biting mosquitoes were collected by human bait and pyrethrum knockdown methods. Sporozoite index was determined with Enzyme-linked Immunosorbent Assays (ELISA). Molecular characterization of sibling species of An. gambiae complex was done with Polymerase Chain Reaction (PCR).A total of 211 An. gambiae complex mosquitoes were collected among which 80(38.0%) were outdoor biting adults while 131(62.0%) were indoor biting adults. There was a significant difference in the number of An. gambiae mosquitoes collected indoors and outdoors (P<0.05, P value=0.000). Out of 150 An. gambiae complex mosquitoes randomly selected, 124(82.67%) were An. gambiaes.s while 26(17.33%) could not be identified. Only 5(10%) out of 50 randomly selected were positive for sporozoites. These observations indicate that malaria transmission in Awka is mainly by An. gambiaes.s.
Malaria sporozoites rate of the bloodfed <em>Anopheles gambiae</em> by Enzyme-linked Immunosorbent Assay (ELISA)
Fig.: Malaria sporozoites rate of the bloodfed Anopheles gambiae by Enzyme-linked Immunosorbent Assay (ELISA)
Pages: 96-101  |  589 Views  15 Downloads
How to cite this article:
Irikannu KC, Onyido AE, Umeanaeto PU, Onwube O, Ogaraku JC, Egbuche CM, Oyeniyi AT, Adesalu OO, Jimoh TR. Molecular characterization and malaria transmission potential of Anopheles gambiae complex in Awka, Anambra state, Nigeria. Int J Mosq Res 2019;6(6):96-101.
International Journal of Mosquito Research