Molecular characterization of mosquito in eastern Tripoli, Libya for species Culex pipiens, molestus and hybrids
Author(s): Hanan A Aqeehal, Hanan M Abuabaid, Walid K Saadawi and Badereddin B Annajar
Abstract: Our study provides a molecular tool for identification of both Culex pipiens forms and their hybrids at Souq Al-jum’aa municipality east of Tripoli district / Libya. Notably, the two forms are morphologically indistinguishable but differ extensively in behavior and physiology and have different epidemiologic importance. Mosquito samples were collected during 2016 from 10 sites within six months; molecular analysis preceded using primers designed for the flanking region of microsatellite CQ11 to identify the two forms of Culex pipiens: and molestus compared to their hybrids. The genetic analysis of collected samples indicated that Culex pipiens species had the highest distribution among the other species at percentage of 51% followed by Culex molestus at 35%, and 14% for the Hybrids. This molecular evidence was confirmative tool to establish the presence of both forms of Cx. pipiens and distinguish them between the hybrids in Souq Al-jum’aa / Tripoli district.
2% Agarose gel electrophoresis of PCR product from the flanking region of the CQ 11 microsatellite of Cx. pipiens, fragments amplified with pipCQ11R, molCQ11R, and CQ11F2 specific primers. The lane M= Marker (size standard 100-bp ladder), lane 1= Negative control, 2 = Cx. pipiens f. pipiens
, lane 2 = Cx. pipiens f. molestus
, lane 3 = Cx. p. pipiens/
molestus hybrids, lane 4 = Positive control
How to cite this article:
Hanan A Aqeehal, Hanan M Abuabaid, Walid K Saadawi, Badereddin B Annajar. Molecular characterization of mosquito in eastern Tripoli, Libya for species Culex pipiens, molestus and hybrids. Int J Mosq Res 2019;6(6):26-30.