Vol. 4, Issue 5, Part A (2017)
Ovitrap survey and serotype identification of dengue virus on Aedes sp mosquito in Potorono, Banguntapan, Bantul, Indonesia
Author(s): Irena Agustiningtyas and Novyan Lusiyana
Abstract: Dengue fever is caused by DENV transmitted by Aedes sp. Ovitrap is used to survey the density of Aedes sp population. The aim of the study was to survey the adult p of Aedes sp and to identify DENV serotype. Ovitraps were used to collect the eggs of Aedes sp in indoor and outdoor of randomly selected houses. Total of 100 ovitraps was provided in 4 areas, 95 ovitraps were collected after nine days. Dengue virus serotypes were determined by RT-PCR. Ovitrap index in RT 7, RT 13, RT 1, and RT 3 were 42,9%, 64%, 54.2%, and 20%, respectively. The results of RT-PCR in 4 areas showed negative DENV 1-4. Ovitrap index in 4 areas of Potorono, Banguntapan, Bantul is high with the most abundant mosquitoes was Aedes aegypti. Surveillance of adult Aedes sp mosquitos is necessary for vector management control of DENV transmission.
The result of RT-PCR using Lanciotti primers to detect serotype of Dengue virus in adult mosquitoes from 4 areas in Potorono, Banguntapan, Bantul on agarose gel 2%. Line 1: Marker 100 bp DNA ladder: line 2: RT 1 negative; line 3: RT 3: negative; line 4: RT 7: negative; line 5: negative; line 6: positive control DEN 3
How to cite this article:
Irena Agustiningtyas, Novyan Lusiyana. Ovitrap survey and serotype identification of dengue virus on Aedes sp mosquito in Potorono, Banguntapan, Bantul, Indonesia. Int J Mosq Res 2017;4(5):32-37.