Morphometry and Phylogeny reconstruction Aedes sp. based DNA Mitochondrial cytochrome oxidase gene sub unit 1 (CO1) in North Sulawesi
Author(s): Stevanus Timah and Mokosuli Yermia Semuel
Abstract: Aedes sp. known as the mosquito vector of dengue viruses that cause dengue fever disease. North Sulawesi is one of the areas with high cases of dengue fever in Indonesia. Present research was conducted to get the morphological characteristics of Aedes sp. in North Sulawesi and to construct phylogenies Aedes sp. of North Sulawesi based on partial CO1 gene. Samples Aedes sp. obtained from Bitung, North Minahasa, Minahasa, South Minahasa, Kotamobagu, Bolaang Mongondow and Sanger. Location mosquito samples, determined by random purposive sampling method. A total of 15 individual samples of adult mosquitoes every origin, used for the analysis of 16 morphological characters and DNA analysis of CO1 gene. Morphometric measurements performed using Stereomicroscope KH8700 mosquito digital 3-D, equipped with a camera and software measurement. Measurement data, subsequent cluster analysis method of principle component analysis, to get a cluster or group Aedes, which has the highest degree of similarity. Molecular characteristics Aedes sp., is determined using CO1 gene. DNA extraction, then used as a template for CO1 gene amplification by PCR. The results of this research showed, that has been found morphometry variation of Aedes sp. from various regions in North Sulawesi. From phylogeny tree is formed, showed that the four samples of mosquitoes (BM = Bolaang Mongondow, Bt = Bitung, KTG = Kotamobagu and Sa = Sanger) based CO1 gene sequences, have the closest relationship with the phylogeny of Aedes albopictus.
Elektrogram CO1 gene amplification of Aedes
CO1 gene is read on the 415 bp to 451 bp (M = marker 1000bp, 1 = Bm, 2 = Bt, 3 = Mktg, 4 = Sa)
How to cite this article:
Stevanus Timah, Mokosuli Yermia Semuel. Morphometry and Phylogeny reconstruction Aedes sp. based DNA Mitochondrial cytochrome oxidase gene sub unit 1 (CO1) in North Sulawesi. Int J Mosq Res 2017;4(3):98-106.