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International Journal of Mosquito Research
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International Journal of Mosquito Research
Vol. 4, Issue 2, Part A (2017)

Molecular characterization of vitellogenesis in anautogenous Culex pipiens pipiens L. mosquitoes

<em>Culex quinquefasciatus</em> Vtg gene sequence diagram showing the intron-exon structure and the positions of the designed Vtg-specific primers (VgF, VgR1, VgR2) used for amplification of Vtg homologue(s) from in <em>Cx. pipiens </em>collected from Egypt. E: exon region.
Fig. 1: Culex quinquefasciatus Vtg gene sequence diagram showing the intron-exon structure and the positions of the designed Vtg-specific primers (VgF, VgR1, VgR2) used for amplification of Vtg homologue(s) from in Cx. pipiens collected from Egypt. E: exon region.
CLUSTAL W multiple alignments at the Vtg nucleotide level of the sequence contig, for <em>Cx. pipiens </em>females 48 h PBM, with four Vg-A1 transcripts in <em>Cx. quinquefasciatus</em>
Fig. 2: CLUSTAL W multiple alignments at the Vtg nucleotide level of the sequence contig, for Cx. pipiens females 48 h PBM, with four Vg-A1 transcripts in Cx. quinquefasciatus
CLUSTAL W multiple alignments at the Vtg nucleotide level of the sequence contig, for <em>Cx. pipiens </em>females 48 h PBM, with four Vg-A1 transcripts in <em>Cx. quinquefasciatus</em>
Fig. 3: CLUSTAL W multiple alignments at the Vtg nucleotide level of the sequence contig, for Cx. pipiens females 48 h PBM, with four Vg-A1 transcripts in Cx. quinquefasciatus
CLUSTAL W multiple alignments at the Vtg nucleotide level of the sequence contig, for <em>Cx. pipiens </em>females 48 h PBM, with four Vg-A1 transcripts in <em>Cx. quinquefasciatus</em>
Fig. 4: CLUSTAL W multiple alignments at the Vtg nucleotide level of the sequence contig, for Cx. pipiens females 48 h PBM, with four Vg-A1 transcripts in Cx. quinquefasciatus
CLUSTAL W multiple alignments at the Vtg nucleotide level of the sequence contig, for <em>Cx. pipiens </em>females 48 h PBM, with four Vg-A1 transcripts in <em>Cx. quinquefasciatus</em>
Fig. 5: CLUSTAL W multiple alignments at the Vtg nucleotide level of the sequence contig, for Cx. pipiens females 48 h PBM, with four Vg-A1 transcripts in Cx. quinquefasciatus
Phylogenetic analysis of Vtg gene transcripts from various species of mosquitoes. The analysis involved 10 nucleotide sequences. All positions containing gaps and missing data were eliminated. There were a total of 39 positions in the final dataset
Fig. 6: Phylogenetic analysis of Vtg gene transcripts from various species of mosquitoes. The analysis involved 10 nucleotide sequences. All positions containing gaps and missing data were eliminated. There were a total of 39 positions in the final dataset
Quantitative expression of <em>Cx. pipiens</em> Vtg gene in immatures and adult mosquitoes at different feeding status. Each point represents the average of three replicates. Bars denote to the standard error
Fig. 7: Quantitative expression of Cx. pipiens Vtg gene in immatures and adult mosquitoes at different feeding status. Each point represents the average of three replicates. Bars denote to the standard error
SDS-PAGE of total Coomassie Brilliant Blue stained ovarian protein extract (20 mg/ ml). SF: sugar-fed females, 0: immediately PBM, 24 h PBM, 48 h PBM, 72 h PBM, 96 h PBM, M: protein ladder (KDa)
Fig. 8: SDS-PAGE of total Coomassie Brilliant Blue stained ovarian protein extract (20 mg/ ml). SF: sugar-fed females, 0: immediately PBM, 24 h PBM, 48 h PBM, 72 h PBM, 96 h PBM, M: protein ladder (KDa)
International Journal of Mosquito Research