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International Journal of Mosquito Research
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International Journal of Mosquito Research
Vol. 2, Issue 2, Part A (2015)

Impact of adult age on forensic use of Culex pipiens mosquito (Diptera: Culicidae)

Author(s): Ahmed M. A. Ibrahim
Abstract: Mosquitoes feed on blood which is a key marker in forensic applications. We identified the impact of female Culex pipiens age on person identification using Short Tandem Repeats analysis (STRs) via blood present in their abdomen and established a correlation between mosquito age and Red Blood Cells (RBCs) degradation as indicator about time passed after blood feeding. Human DNA from adults was reduced in samples from 0 and 2 days old than 4-10 days old. These samples failed to supply full STRs profiles (0 and 2 days old females supplied 11 and 10 sites). Older females supplied all of the 14 STRs. Temporal count of RBCs from females with different ages after mosquito feeding revealed faster degradation of RBCs in blood drawn from older females (4-10 days old) than those of younger samples (0 and 2 days old). Age of mosquito has a key impact on forensic use of mosquitoes.
Effect of adult female <em>Culex</em> age on temporal pattern of degradation of RBCs in human blood drawn from mosquito with adult age (F) 10 days. After blood feeding for 30 minutes, mosquitoes were cultured in 28±1 °C and RBCs were counted at 0 (immediate count)48 and 72 hour after blood ingestion. Error bars represent standard deviations of three measurements. Different letters above error bars represent significant difference among means at type error = 0.05 (ANOVA)
Fig.: Effect of adult female Culex age on temporal pattern of degradation of RBCs in human blood drawn from mosquito with adult age (F) 10 days. After blood feeding for 30 minutes, mosquitoes were cultured in 28±1 °C and RBCs were counted at 0 (immediate count)48 and 72 hour after blood ingestion. Error bars represent standard deviations of three measurements. Different letters above error bars represent significant difference among means at type error = 0.05 (ANOVA)
Pages: 28-32  |  1097 Views  22 Downloads
How to cite this article:
Ahmed M. A. Ibrahim. Impact of adult age on forensic use of Culex pipiens mosquito (Diptera: Culicidae). Int J Mosq Res 2015;2(2):28-32.
International Journal of Mosquito Research