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International Journal of Mosquito Research
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International Journal of Mosquito Research
Vol. 1, Issue 4, Part A (2014)

Structural and functional prediction analysis of mosquito Ninjurin protein: Implication in the innate immune responses in Anopheles stephensi

<strong>3D- <em>In silico</em> analysis of mosquito Ninjurin: </strong>(a) Secondary structure prediction of <em>AsNinj</em>L; (b) Ramachandran Plot analysis; (c) Prosa energy distribution of the molecular modeled mosquito Ninjurin; (d, e) Surface representation of the Ninjurin: two front views (d) one side view (e). Polar, negatively and positively charged atoms are shown in green, red and blue respectively; (g) STRING based network prediction analysis of <em>AsNinj </em>L
Fig. 1: 3D- In silico analysis of mosquito Ninjurin: (a) Secondary structure prediction of AsNinjL; (b) Ramachandran Plot analysis; (c) Prosa energy distribution of the molecular modeled mosquito Ninjurin; (d, e) Surface representation of the Ninjurin: two front views (d) one side view (e). Polar, negatively and positively charged atoms are shown in green, red and blue respectively; (g) STRING based network prediction analysis of AsNinj L
Genomic and molecular Gene Organization of mosquito Ninjurin: (a) Schematic representation of the genomic architecture of the mosquito <em>Anopheles stephensi</em> ninjurins. Grey regions indicates the; predicted transmembrane of the ninjurin domain, and +1 mark; the; transcription initiation site; (b) Gene organization and molecular features of complete CDS; of <em>AsNinjL</em>: The gene contains 1854 bp, encoding 423 AA long peptides with two ninjurin domains, whereas <em>AsNinj S</em> contains only one ninjurin domain.  Both 5’ and 3’-UTR regions are shown in bold & capital letters. The complete CDS region of 423 amino acids starts from ATG/Methionine/green color, ending with TGA/Red/*. The different predicted motifs viz. N Glycosylation site (dark blue); PKC (Pink); CKP (sky blue); cAMP phosphorylation site(dark yellow arrow); Amidation site (dark red); Mrystoylation site (green dotted) are underlined. Other additional structural features like transmembrane domains are underlined by dark grey lines; (c) Phylogenetic relation of mosquito and insect ninjurins. AsNinjL & AsNinjS represents longer (ASTE010358) and smaller (ASTEI08643) <em>Anopheles stephensi </em>ninjurin transcripts, respectively; E1-E5: no. of exons; UTR: Untranslated region
Fig. 2: Genomic and molecular Gene Organization of mosquito Ninjurin: (a) Schematic representation of the genomic architecture of the mosquito Anopheles stephensi ninjurins. Grey regions indicates the; predicted transmembrane of the ninjurin domain, and +1 mark; the; transcription initiation site; (b) Gene organization and molecular features of complete CDS; of AsNinjL: The gene contains 1854 bp, encoding 423 AA long peptides with two ninjurin domains, whereas AsNinj S contains only one ninjurin domain. Both 5’ and 3’-UTR regions are shown in bold & capital letters. The complete CDS region of 423 amino acids starts from ATG/Methionine/green color, ending with TGA/Red/*. The different predicted motifs viz. N Glycosylation site (dark blue); PKC (Pink); CKP (sky blue); cAMP phosphorylation site(dark yellow arrow); Amidation site (dark red); Mrystoylation site (green dotted) are underlined. Other additional structural features like transmembrane domains are underlined by dark grey lines; (c) Phylogenetic relation of mosquito and insect ninjurins. AsNinjL & AsNinjS represents longer (ASTE010358) and smaller (ASTEI08643) Anopheles stephensi ninjurin transcripts, respectively; E1-E5: no. of exons; UTR: Untranslated region
Transcriptional behavior of mosquito ninjurin protein:<strong> </strong>(a) <em>AsNinj</em> expression during the development of mosquito. Developmental stages include Egg, Larva, pupa, adult female; M= 500bp Ladder; (b) Tissue specific relative expression of <em>AsNinj</em>, HC: Hemocyte; MG: Midgut; FB: Fat body; (c) Injury specific response of (PBS: phosphate buffer saline); (d) Up regulation of <em>AsNinj</em> in response to microbial challenge.
Fig. 3: Transcriptional behavior of mosquito ninjurin protein: (a) AsNinj expression during the development of mosquito. Developmental stages include Egg, Larva, pupa, adult female; M= 500bp Ladder; (b) Tissue specific relative expression of AsNinj, HC: Hemocyte; MG: Midgut; FB: Fat body; (c) Injury specific response of (PBS: phosphate buffer saline); (d) Up regulation of AsNinj in response to microbial challenge.
International Journal of Mosquito Research