Novel techniques for cost-effective production of Bacillus thuringiensis Subsp. israelensis

Authors: C Gopinathan, Arindam Chaudhury and AT Vivek
Bacillus thuringiensis is a desirable biopesticide because of its low cost and lack of toxicity. The use of Bt in developing countries is limited due to process complications and high cost of production. In the present study, we have shown the fed-batch fermentation method using inexpensive alternatives for carbon and protein sources can effectively reduce the cost in large-scale production of Bti. We have shown that with the alternative media supplements, neither the biomass production, nor larvicidal activity is hampered. In addition, we have also shown the positive effects of sparged aeration, and the indispensable role of yeast extract. We have provided the first experimental proof delineating the sporulation process and delta-endotoxin production. We have investigated the role of various buffering agents and additives in increasing biomass production and early sporulation.

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