Engineering lhRNA based molecule to interfere replication of dengue virus in transgenic Aedes aegypti mosquitoes: Bioinformatics approach

Authors: Sujeewa Sampath LD, Ranil Samantha Dassanayake, Kajan M, YI Nilmini Silva Gunawardena
As a preliminary step towards the development of transgenic Aedes aegypti mosquito’s resistance to Sri Lankan dengue viruses (SLDENV), the identification of previously unpublished DNA sequence of the construct, AeCPA/Mnp+/i/Mnp-/svA, was done using bioinformatics tools. Furthermore, a long hairpin RNA (lhRNA) molecule effective against all four SLDENV serotypes was designed. If this lhRNA molecule to be cleaved by dicer at the correct site, the 100% of siRNA molecules produced are going to be effective against each serotype of SLDENV, on the contrary, if it is to be cleaved at random sites, which is the worst case, 52.63% of total siRNA molecules produced are going to be effective against each SLDENV serotype. In addition, an alternative promoter sequence, Ae. aegypti vitellogenin A1 (AeVtA), and a construct functionally similar to MosI helper plasmid, pKhsp82MOS (commercially unavailable) used in micro injection procedure of Ae. aegypti were also proposed in this study.

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