Structural and functional prediction analysis of mosquito Ninjurin protein: Implication in the innate immune responses in Anopheles stephensi

Authors: Tina Thomas, Tanwee Das De, Punita Sharma, Sonia Verma, Suman Rohilla, Kailash C Pandey, Rajnikant Dixit

Ninjurin, identified as a two-pass transmembrane protein is induced upon nerve injury in vertebrates.  Recent studies demonstrate that ninjurins are cell adhesion molecules, capable of regulating many cellular functions viz. embryogenesis, injury, inflammation, signals etc. However, their structural and functional properties controlling these cellular responses, especially innate immune responses have not been investigated in detail. Through comprehensive molecular and functional genomics approach, here we characterize and predict the possible role of mosquito ninjurin (AsNinj) in hemocyte mediated cellular immune response. Molecular modeling analyses provide crucial information about the key residues of ninjurin proteins, for further in vitro and in vivo functional analysis.

 

Fig:3D- In silico analysis of mosquito Ninjurin: (a) Secondary structure prediction of AsNinjL; (b) Ramachandran Plot analysis; (c) Prosa energy distribution of the molecular modeled mosquito Ninjurin; (d,e) Surface representation of the Ninjurin: two front views (d) one side view (e). Polar, negatively and positively charged atoms are shown in green, red and blue respectively; (g) STRING based network prediction analysis of AsNinjL

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