A comparative study on bio-efficacy and wash resistance of five long lasting insecticidal mosquito nets against malaria vector Anopheles culicifacies

Ripu Daman Sood, Neera Kapoor, P.K. Mittal

The study was carried out to test the wash resistance and bio-efficacy of five long lasting insecticidal nets (LLINs) namely Olyset^® Net, PermaNet^® 2.0, Icon Life^®, Duranet^®, and Interceptor^® net against Anopheles culicifacies, a major rural malaria vector in India. Mortality and median knockdown time (MKDT) of mosquitoes on five different LLINs, fresh and washed up to 20 times, were determined by using cone bioassays and ring net bioassays, respectively. LLINs showed high efficacy against An. culicifacies. The mortality of mosquitoes remained >80% even after 20 hand washes on all the five LLINs tested. There was a significant difference in MKDT of An. culicifacies on different LLINs. MKDT increased progressively with successive washes. The study revealed that all the LLINs were effective to produce>80% mortality against An. culicifacies even after 20 washes. However, there was a significant difference in MKDT between fresh nets and 20 times washed nets in case of all the five LLINs. After 20 washes, the lowest MKDT was observed on PermaNet®2.0, while the highest MKDT was observed on Interceptor^® net.

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