Vol. 4, Issue 6, Part A (2017)

Fig. 1: 15% native-PAGE (Stained with Coomassie Brilliant Blue) of salivary glands protein of Culex pipiens male and female at different times of feeding. L1: 3 h post-sugar-feeding male, L2: 3 h post-sugar-feeding female, L3: 12 h post-sugar-feeding male, L4: 12 h post-sugar-feeding female, L5: 24 h post-sugar-feeding male, L6: 24 h post-sugar-feeding female, L7: 48 h post-sugar-feeding male, L8: 48 h post-sugar-feeding female, L9: 72 h post-sugar-feeding male, L10: 72 h post-sugar-feeding female and L11: Starved male.

Fig. 2: 15% native-PAGE (Stained with Coomassie Brilliant Blue) of salivary glands protein of Culex pipiens male and female at different times of feeding. L1: Starved female, L2: un-fed male, L3: un-fed female, L4: Skin exploring female, L5: 3 h post-blood-feeding female, L6: 12 h post-blood-feeding female, L7: 24 h post-blood-feeding female, L8: 48 h post-blood-feeding female, L9: 72 h post-blood-feeding female and L10: post-oviposition female.

Fig. 3: 15% SDS-PAGE (Stained with Coomassie Brilliant Blue) of salivary glands protein of Culex pipiens male and female at different times of feeding. L1: 3 h post-sugar-feeding male, L2: 3 h post-sugar-feeding female, L3: 12 h post-sugar-feeding male, L4:12 h post-sugar-feeding female, L5: 24 h post-sugar-feeding male, L6: 24 h post-sugar-feeding female, L7: 48 h post-sugar-feeding male, L8: 48 h post-sugar-feeding female, L9: 72 h post-sugar-feeding male, L10: 72 h post-sugar-feeding female and lane M: Size of molecular weight marker is indicated on the left.

Fig. 4: 15% SDS-PAGE (Stained with Coomassie Brilliant Blue) of salivary glands protein of Culex pipiens male and female at different times of feeding. L1: Starved male, L2: Starved female, L3: un-fed male, L4: un-fed female, L5: Skin exploring female, L6: 3 h post-blood-feeding female, L7: 12 h post-blood-feeding female, L8: 24 h post-blood-feeding female, L9: 48 h post-blood-feeding female, L10: 72 h post-blood-feeding, L11: post-oviposition female and lane M: Size of molecular weight marker is indicated on the left.

Fig. 5: Number of protein bands generated by the SDS-PAGE in sugar-fed, starved, un-fed males and females, skin exploring, blood-fed and post-oviposition for female only.