Abstract: Aims & objectives:
To study the biochemical and hematological parameters of blood of patients suffering from different levels of severity of falciparum malaria and proteomic analysis of these patients. The ultimate objective of this research plan was to utilize the proteomic platform for discovery of falciparum malaria biomarkers for diagnosis of asymptomatic and low-parasitemic falciparum malaria patients.
Material and Methods: After implication of inclusion and exclusion criteria, 52 cases were selected for study, out of which 17 cases were in severe category and 35 were non severe. Comparative proteomic analysis of malaria and controls, Immunoassay-based validations, Proteins networks and functional analysis, and PCR confirmation of diagnosis was done. The comprehensive serum proteome analysis was done on these samples using 2D-Electrophoresis.
Results: This study has identified a number of differentially expressed serum proteins, 5 proteins were found to be significant between healthy and non-severe malaria serum samples, 5 between healthy and severe malaria serum samples and 3 between severe and non-severe serum samples.
Conclusion: Differentially expressed proteins in the context of P. falciparum malaria will be valuable in understanding the basis for the disease pathogenesis and may ultimately help to establish potential surrogate markers aiding in diagnosis, prognosis, monitoring disease progression.