Suitability of the boiling method of DNA extraction in mosquitoes for routine molecular analyses

D. K. Sarma; S. Singh; D. R. Bhattacharyya; P. K. Mohapatra; N. P. Sarma; G. U. Ahmed; J. Mahanta; Anil Prakash

Vol. 1, Issue 3, Part A (2014)

Suitability of the boiling method of DNA extraction in mosquitoes for routine molecular analyses

Author(s): D. K. Sarma, S. Singh, D. R. Bhattacharyya, P. K. Mohapatra, N. P. Sarma, G. U. Ahmed, J. Mahanta, Anil Prakash

Abstract: This communication deals with the experience on suitability of the boiling method of DNA extraction from mosquito tissues. The DNA extracted by this method was found, by and large, stable after 30 months of storage. The method is useful for routine molecular entomological applications.

Gel images of the amplified PCR products for different nuclear and mitochondrial loci of <em>An. baimaii</em> DNA extracted using boiling method (lanes 2 & 3 fresh DNA, lanes 4 & 5 DNA stored for 30 months.

Fig.: Gel images of the amplified PCR products for different nuclear and mitochondrial loci of An. baimaii DNA extracted using boiling method (lanes 2 & 3 fresh DNA, lanes 4 & 5 DNA stored for 30 months.

Pages: 15-17 | 1968 Views 120 Downloads

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How to cite this article:

D. K. Sarma, S. Singh, D. R. Bhattacharyya, P. K. Mohapatra, N. P. Sarma, G. U. Ahmed, J. Mahanta, Anil Prakash. Suitability of the boiling method of DNA extraction in mosquitoes for routine molecular analyses. Int J Mosq Res 2014;1(3):15-17.

Journal's Code

ISSN Number: 2348-5906 (Online)
ISSN Number: 2348-7941 (Print)
CODEN Code: IJMRK2
ZDB-Number: 2763847-9
Abbreviated Title: Int. J. Mosq. Res.
Indexed journal: Yes
Refereed Journal: Yes

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Vol. 1, Issue 3, Part A (2014)

Suitability of the boiling method of DNA extraction in mosquitoes for routine molecular analyses

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